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MPHL : Arbovirus and Virus Isolation

 

 Arbovirus and Virus Isolation Laboratory

 

Laboratory Scientist Supervisor: 
Heather Peters
410-767-6153
     
Laboratory Scientist Lead:
Benjamin Healey
410-767-6166 

Arbovirus Serology
 
 
Arbovirus Serology Laboratory: 410-767-6153/ 410-767-3942
 
The Arbovirus Serology Laboratory performs IgM testing on human serum and cerebrospinal fluid (CSF) for West Nile Virus (WNV), St. Louis Encephalitis (SLE), and Eastern Equine Encephalitis (EEE). Lacrosse (LAC) IgM testing is available based on patient’s travel history.  Dengue Fever IgM testing is available on serum samples for patient’s with pertinent travel history. Refer to the CDC guidelines on diagnosis of Dengue Fever. All human arboviral test requests must indicate the specimen collection date, patient’s onset of symptoms, yellow fever vaccination history, and patient’s travel history. Please refer to “Arbovirus Serological Testing Labslip Training Document and the Arbovirus Serology Specimen Submission Guidelines” links below for further guidance. Contact 410-767-6120 to acquire laboratory requisition forms.
 
Testing for other arboviruses are available at the CDC. All samples should be submitted to the State of MD DHMH Laboratory using the human arboviral submission guidelines referenced below. Some of the tests available are: Japanese Encephalitis, Chikungunya (CHIK), Powassen (POW/TBE), Yellow Fever (YF), Western Equine Encephalitis (WEE), and Venezuelan Equine Encephalitis (VEE). CDC requires all human arboviral test requests to indicate the specimen collection date, patient’s onset of symptoms, yellow fever vaccination history, and patient’s travel history.
 
 
 
 
 
Equine Arbovirus Testing 
 
From July 1 – October 31 of each year, the Laboratories Administration will accept serum, brain, and/or CSF samples from horses exhibiting clinical signs consistent with arboviral disease for testing. A veterinarian wishing to request arboviral testing of equine specimens should first contact the Center for Zoonotic and Vector-borne Diseases (CZVBD) at
410-767-5649 to coordinate submission of specimens. An Equine Arboviral Testing Form must accompany each submission.

West Nile IgM testing is performed on Equine serum and cerebrospinal fluid (CSF). Please refer to the “West Nile IgM Equine Specimen Submission Guidelines” link below for further guidance.
 
 
 
  
 Virus Isolation 
 
Virus Isolation Laboratory: 410-767-6153 
 
The Virus Isolation Laboratory performs culture testing for the following viruses: Adenovirus, Cytomegalovirus (CMV), Enterovirus (including Echovirus, Coxsackie, and Polio), Herpes Simplex Virus (HSV Types 1 & 2), Influenza (Types A & B), Measles, Mumps, Parainfluenza (Types 1,2 & 3), Respiratory Syncytial Virus (RSV), and Varicella Zoster Virus (VZV).
 
Contact 410-767-6120 to acquire laboratory requisition forms.