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MPHL Home : Gonnorhea Laboratory

Gonococcal (GC) Laboratory 
 
Laboratory Scientist Lead:
Mara Johnson
410-767-6132 

The GC Laboratory tests genital and extra general specimen sources of Neisseria gonorrhoeae using the appropriate culture media submitted by health care providers.  We test oxidase positive, catalse positive, gram negative diploccoci by DNA probe.  Positive genital specimens are reported without further testing.  Extra genital, ASA and children 13 years old and under require additional testing to confirm a positive test for Neisseria gonorrhoeae.  All positive tests are then set up for susceptibility testing using six (6) ant-microbials.  Susceptibility testing is reported upon request, if extra-genital, ASA, and children 13 years old and under.  Some specimens are referred to CDC is resistance is detected.
 
Frequently Asked Questions include:
 
  • Can we submit a plate on expired media?
The collection of the specimen must be before or on the day of expiration of the media to ensure best recovery.
 
  • We missed the courier.  Is it okay to still send the specimen?
We must receive the specimen within four (4) days of collection.  If collected in Amies, we must receive the specimen within 24 hours.
 
  • What media can we use to collect specimen for submission for gonorrhoeae testing?
Please submit on GC-Lect (MTM) for best results.  Chocolate or Amies can also be submitted.  However, there is a greater chance of other organisms growing before Neisseria gonorrhoeae has a chance to establish growth.
 
  • Do we have to sue a CO2 tablet?
CO2 is one of the growth requirements for Neisseria gonorrhoeae.  A tablet must be included with each plate submitted.  This tablet should remain in the foil packet, tearing or cutting a corner to allow exposure to the air.